(a) Do you think MBB1 can form disulphide bonds. Explain your answer. (3 marks, 3 lines)
(b) Which residues of MBB1 could be post-translationally modified? In your answer, state the residues and the post-translational modifications. (4 marks, 4 lines)
(c) At physiological pH, what do you think the overall charge of MBB1 will be? Assume that the N-terminus and C-terminus of MBB1 are protonated and deprotonated respectively (-NH3+ and -COO-). Show your full method of working out of your answer. (5 marks, 6 lines)
The tertiary structure of MBB1 is shown below:
(d) Describe the tertiary structure of MBB1. (2 marks, 2 lines)
(e) The crystal structure of purified MBB1 found that the protein exists as a homohexameric complex. How many amino acids would you expect to find in this MBB complex? Show your full method of working out of your answer.
(2 marks, 2 lines)
