Give general background for the purpose of experiment. What is your hypothesis? Why is it important to identify your unknown organism? Be sure to introduce EACH test or stain, what it tells you about the microorganism, and justification for utilizing it .
Discuss the aseptic techniques you used throughout the experiment. This section should be written in past tense, since it is something you have already done! How did you get the bacteria in order to perform inoculate the tests? Streak plate? Broth?
After reading your paper, someone with the exact same unknown bacteria should be able to follow your methods and come out with the same results.
List all reagents used and how much you used for each test conducted. You MUST mention EVERY test you performed! Even if the test produced a negative result.
