Antimicrobial Susceptibility Test on Unknown Bacterium
1. Click on this link, and read up on the theory behind and the protocol for performing a Kirby Bauer Antibiotic Sensitivity Assay https:
2. Review the given antibiotic sensitivity results for each of your unknowns. Please understand that I could not find all identical assays, performed with the same antibiotics.
3. Your assignment this week is to:
a. Finish your results section, with the antibiotic sensitivity results from this week included
i. I understand that some of these results are in diameter of zone of inhibition, some report S = susceptible or NS = not susceptible, some report % resist, and others a photo of the zones of inhibition
ii. The goal here is not to have perfect results but, rather, to help support what you know about if your unknown is gram positive or gram-negative, and how its biochemical characteristics from the past couple week’s tests.
For example, if you have Unknown C/3, you know it is Gram-negative; therefore, would you expect it to be susceptible to penicillin? Why or why not?
