Why was this done, and what do the results suggest about the role of the biotin moeity with respect to ribozyme activity?
1) Included in the analysis of the ribozyme kinetic data are Michaelis constants and catalysis rate constants. How do the values for the Diels-Alderase ribozyme compare to protein enzymes found in living organisms? For reference, it may be helpful to consult Lecture 2-8 slide 16. 2) Part of the investigation included an examination of different […]